Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA136789

Local Sample ID:	YGR023w-4
Subject ID:	SU001689
Subject Type:	Yeast
Subject Species:	Saccharomyces cerevisiae
Taxonomy ID:	4932
Genotype Strain:	BY4742, YGR023w
Cell Biosource Or Supplier:	Open Biosystems

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Sample Preparation:

Sampleprep ID:	SP001695
Sampleprep Summary:	Extraction of metabolites was performed by homogenization in 1 mL of cold methanol/H2O (1:1) extraction solution and centrifugated at 167 x g at 4°C for 5 min. Supernatants were collected and evaporated to dryness in a nitrogen stream stream at 50 ºC (RapidVap, Labconco), and derivatized by methoxyamination by adding 50 μl of 20 mg/ml solution of methoxyamine hydrochloride in pyridine followed by incubation at 37°C for 2 hours. Trimethylsilylation was subsequently performed by adding 50 µl of N-methyl-N-trimethylsilyl-trifluoroacetamide (MSTFA+1% TMCS) and incubated for 1 hour at 65°C. Samples were centrifuged at 13000 rpm for 10 minutes at RT. Supernatants were transferred to glass vials for GC/MS analysis

Sampleprep ID:

SP001695

Sampleprep Summary:

Extraction of metabolites was performed by homogenization in 1 mL of cold methanol/H2O (1:1) extraction solution and centrifugated at 167 x g at 4°C for 5 min. Supernatants were collected and evaporated to dryness in a nitrogen stream stream at 50 ºC (RapidVap, Labconco), and derivatized by methoxyamination by adding 50 μl of 20 mg/ml solution of methoxyamine hydrochloride in pyridine followed by incubation at 37°C for 2 hours. Trimethylsilylation was subsequently performed by adding 50 µl of N-methyl-N-trimethylsilyl-trifluoroacetamide (MSTFA+1% TMCS) and incubated for 1 hour at 65°C. Samples were centrifuged at 13000 rpm for 10 minutes at RT. Supernatants were transferred to glass vials for GC/MS analysis