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MB Sample ID: SA137920
| Local Sample ID: | F2 |
| Subject ID: | SU001698 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
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Sample Preparation:
| Sampleprep ID: | SP001704 |
| Sampleprep Summary: | To harvest intracellular metabolites, organoid samples were washed with pre-cooled PBS for three times and metabolism was quenched with 80% ice-cold methanol. Pre-cooled methanol (1mL) with para-chlorophenylalanine (1 µM) as the internal standard was added to each sample and incubated on ice for 10 min. Sample lysates were then centrifuged at 4 °C for 15 min at 23,000 g. |
