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MB Sample ID: SA151683
| Local Sample ID: | A26 |
| Subject ID: | SU001726 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 4.14 to 8.86 years |
| Weight Or Weight Range: | 4.5 to 41.3 kg |
| Height Or Height Range: | 56.0 to 143.1 inch |
| Gender: | Male and female |
| Human Race: | white |
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Sample Preparation:
| Sampleprep ID: | SP001732 |
| Sampleprep Summary: | 100 µL of freshly prepared 1 mg/mL urase solution was added to 100 µL of each of the urine samples then vortexed for 20 seconds and allowed to stand at -20 oC for 1 h. Next, 20 µL of 1 mg/mL of ribitol in water was added to each sample followed by addition of 1 mL of MeOH that was precooled to -20 ºC. The solution was again vortexed for 20 seconds and allowed to stand at -20 ºC for another 2 hours. The samples were centrifuged at 13000g for 15 minutes, and 1 ml of the supernatant from each sample was transferred to an autosampler vial and dried under gaseous nitrogen stream. Additionally, a pooled sample was prepared by combining 200 µL of supernatants from each sample and dried under nitrogen. Dried extracts were stored at -20 C until derivatization. |
| Processing Storage Conditions: | -20℃ |
| Extraction Method: | For GCMS-polar analysis samples were extracted with water, containing 1 mg/ml ribitol, and 1 ml methanol. |
| Extract Enrichment: | 1 mL each of supernatant was collected from each sample for GCMS-polar analysis. |
| Extract Storage: | -20℃ |
| Sample Resuspension: | For GCMS analysis extracts dried under nitrogen flow and storred at -20C then resuspended in 25 μl of pyridine containing 15 mg/ml methoxyamine hydrochloride |
| Sample Derivatization: | GCMS samples were derivatized with 25 μl MSTFA (N-methyl-N-(trimethyl-silyl)trifluoroacetamide) + 1% TMCS (chlorotrimethylsilane) |
| Sample Spiking: | Ribitol was used as internal standard for GCMS-polar analysis. |
