Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA152357

Local Sample ID:	C3
Subject ID:	SU001745
Subject Type:	Mammal
Subject Species:	Rattus norvegicus
Taxonomy ID:	10116

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Sample Preparation:

Sampleprep ID:	SP001751
Sampleprep Summary:	For CE-TOFMS, The frozen tissue was plunged into 50% acetonitrile/Milli-Q water containing internal standards. The tissues were homogenized thrice, and then centrifuged. The upper aqueous layer was centrifugally filtered to remove the proteins. The filtrate was then centrifugally concentrated and re-suspended in 50 µL of Milli-Q water for CE-MS analysis. For LC-TOFMS, the frozen tissue was plunged into 1% formic acid/acetonitrile containing the internal standard solution. Tissue was homogenized thrice, and the mixture was yet again homogenized after adding Milli-Q water and then centrifuged. The supernatant was collected and 500 1% formic acid/acetonitrile and Milli-Q water were added to the precipitate. Homogenization and centrifugation were performed as described previously, and the supernatant was mixed with previously collected one. The mixed supernatant was filtrated to remove the proteins and phospholipids. The filtrate was then desiccated and dissolved in 200 µL of isopropanol/Milli-Q water for LC-MS analysis.

Sampleprep ID:

SP001751

Sampleprep Summary:

For CE-TOFMS, The frozen tissue was plunged into 50% acetonitrile/Milli-Q water containing internal standards. The tissues were homogenized thrice, and then centrifuged. The upper aqueous layer was centrifugally filtered to remove the proteins. The filtrate was then centrifugally concentrated and re-suspended in 50 µL of Milli-Q water for CE-MS analysis. For LC-TOFMS, the frozen tissue was plunged into 1% formic acid/acetonitrile containing the internal standard solution. Tissue was homogenized thrice, and the mixture was yet again homogenized after adding Milli-Q water and then centrifuged. The supernatant was collected and 500 1% formic acid/acetonitrile and Milli-Q water were added to the precipitate. Homogenization and centrifugation were performed as described previously, and the supernatant was mixed with previously collected one. The mixed supernatant was filtrated to remove the proteins and phospholipids. The filtrate was then desiccated and dissolved in 200 µL of isopropanol/Milli-Q water for LC-MS analysis.