Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA155402

Local Sample ID:	2287
Subject ID:	SU001761
Subject Type:	Mammal
Subject Species:	Oryctolagus cuniculus
Taxonomy ID:	9986

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Sample Preparation
Chromatography
Analysis
MS

Sample Preparation:

Sampleprep ID:	SP001767
Sampleprep Summary:	The samples were mixed with 1500 µL of 50% acetonitrile in water (v/v) containing internal standards (20 μM) and homogenized by a homogenizer (4500 rpm, 60 sec × 10 times). The supernatant (400 µL) was then filtrated through 5-kDa cut-off filter (ULTRAFREE-MC-PLHCC, Human Metabolome Technologies, Yamagata, Japan) to remove macromolecules. The filtrate was centrifugally concentrated and resuspended in 50 µL of ultrapure water immediately before the measurement.

Sampleprep ID:

SP001767

Sampleprep Summary:

The samples were mixed with 1500 µL of 50% acetonitrile in water (v/v) containing internal standards (20 μM) and homogenized by a homogenizer (4500 rpm, 60 sec × 10 times). The supernatant (400 µL) was then filtrated through 5-kDa cut-off filter (ULTRAFREE-MC-PLHCC, Human Metabolome Technologies, Yamagata, Japan) to remove macromolecules. The filtrate was centrifugally concentrated and resuspended in 50 µL of ultrapure water immediately before the measurement.