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MB Sample ID: SA163734
| Local Sample ID: | trps_Exp1_13_Therapy |
| Subject ID: | SU001825 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | GF 129SvEv background IL-10-deficient mice (Il10-/-); Il10-eGFP-reporter (Il10+/eGFP) mice (on a C57BL/6J background) |
| Age Or Age Range: | 8-12 week old |
| Animal Animal Supplier: | 8-12 week-age GF 129SvEv background IL-10-deficient mice (Il10-/-) were obtained from University of North Carolina National Gnotobiotic Rodent Resource Center; Il10-eGFP-reporter (Il10+/eGFP) mice on a C57BL/6J background mice were originally provided by Dr. C. L. Karp (Global Health, Bill & Melinda Gates Foundation, USA) |
| Animal Housing: | Germ-free and gnotobiotic conditions at National Gnotobiotic Rodent Resource Center, University of North Carolina |
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Sample Preparation:
| Sampleprep ID: | SP001831 |
| Sampleprep Summary: | Bile acids, SCFAs, and tryptophan metabolites were extracted (with careful cleanup or derivatization procedures whenever necessary) and analyzed using mass spectrometry-based platforms. |
| Sampleprep Protocol Filename: | Protocols.pdf |
| Processing Storage Conditions: | On ice |
| Extraction Method: | Bile acid: SPE and LLE; SCFA: alkaline aqueous extraction; tryptophan metabolites: 1:1 MeOH:Water LLE |
| Extract Cleanup: | For Bile acids, C18 solid-phase extraction cleanup and concentration was used. |
| Extract Storage: | -80℃ |
| Sample Derivatization: | For SCFAs, propyl chloroformate derivatization was performed. |
