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MB Sample ID: SA176633

Local Sample ID:	C20
Subject ID:	SU001983
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Sample Preparation:

Sampleprep ID:	SP001989
Sampleprep Summary:	Saliva samples were thawed at 4℃, then vortexed and centrifuged (4 ℃, 18,000 × g) for 3 min. Next, 0.8 mL of the aqueous layer was pipetted off and weighed, then 0.3 mL of that was transferred into a 2-mL glass vial (Nichiden-Rika Glass, Kobe, Japan) and kept at 4℃ in a CubeCooler®. For extraction, 0.3 mL of deaerated MilliQ water containing ribitol (0.02 mg/mL) as an internal standard was added. After incubation using an Eppendorf thermomixer (25℃, 1000 rpm, 10 min), 1.4 mL of deaerated acetonitrile was added. After incubation (25℃, 1000 rpm, 10 min) and centrifugation (4℃, 1800 × g) for 3 min, 1.6 mL of the supernatant was transferred to a 2-mL tube and dried with a vacuum concentrator (VC-96R; TAITEC, Koshigaya, Japan) for 30 min, then allowed to lyophilize overnight. Derivatization was performed with a methoxyamine hydrochloride solution with pyridine at a concentration of 20 mg/mL, followed by silylation application of N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA).

Sampleprep ID:

SP001989

Sampleprep Summary:

Saliva samples were thawed at 4℃, then vortexed and centrifuged (4 ℃, 18,000 × g) for 3 min. Next, 0.8 mL of the aqueous layer was pipetted off and weighed, then 0.3 mL of that was transferred into a 2-mL glass vial (Nichiden-Rika Glass, Kobe, Japan) and kept at 4℃ in a CubeCooler®. For extraction, 0.3 mL of deaerated MilliQ water containing ribitol (0.02 mg/mL) as an internal standard was added. After incubation using an Eppendorf thermomixer (25℃, 1000 rpm, 10 min), 1.4 mL of deaerated acetonitrile was added. After incubation (25℃, 1000 rpm, 10 min) and centrifugation (4℃, 1800 × g) for 3 min, 1.6 mL of the supernatant was transferred to a 2-mL tube and dried with a vacuum concentrator (VC-96R; TAITEC, Koshigaya, Japan) for 30 min, then allowed to lyophilize overnight. Derivatization was performed with a methoxyamine hydrochloride solution with pyridine at a concentration of 20 mg/mL, followed by silylation application of N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA).