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MB Sample ID: SA176768
| Local Sample ID: | 4SR-1 |
| Subject ID: | SU001985 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male |
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Sample Preparation:
| Sampleprep ID: | SP001991 |
| Sampleprep Summary: | Mitochondrial isolates were extracted using a modified single-phase chloroform/methanol extraction as described previously (Weir et al. 2013). In brief, 20 volumes of chloroform:methanol (2:1) were added to the sample along with a series of internal standards. Samples were vortexed and centrifuged on a rotary mixer for 10 min. Following sonication on a sonicator bath for 30 min, samples were rested for 20 min prior to centrifugation at 13,000 g for 10 min. Supernatants were transferred into a 96 well plated, dried down and reconstituted in 50 μL H2O saturated butanol and sonicated for 10 min. After the addition of 50 μL of methanol with 10 mM ammonium 945 formate, samples were centrifuged at 4000 rpm on a plate centrifuge and transferred into glass vials with inserts for mass spectrometry analysis. |
| Processing Storage Conditions: | -80℃ |
| Extraction Method: | Single phase 2:1 chloroform methanol |
| Extract Storage: | -80℃ |
| Sample Resuspension: | Butanol : Methanol 1:1 |
| Subcellular Location: | Mitochondria |
