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MB Sample ID: SA183152

Local Sample ID:20210306-4-100-5
Subject ID:SU002016
Subject Type:Bacteria
Subject Species:Escherichia coli
Taxonomy ID:562

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Sample Preparation:

Sampleprep ID:SP002022
Sampleprep Summary:Each pellet sample was mixed with 2 mL of 80% ice-cold methanol, and the mixed solution was homogenized on ice for 2 minutes and repeated the same procedure for three times. Next, the collected samples were centrifuged at 12000 rpm and 4°C for 15 minutes, to collect the supernatants. The supernatants were further mixed with 800 μL of ice-cold acetonitrile for 20 minutes before further centrifuge at 12000 rpm and 4°C for 15 minutes. At last, the isolated supernatants were gone through a 0.22 μm filter membrane (nylon) before the lyophilization. The lyophilized samples were stored at -80°C for metabolomics analysis.