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MB Sample ID: SA185579
| Local Sample ID: | Pooled Human Plasma 8 |
| Subject ID: | SU002061 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Pooled |
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Sample Preparation:
| Sampleprep ID: | SP002067 |
| Sampleprep Summary: | Four parts of 1:1:1 methanol:acetonitrile:acetone were added to one part human plasma in 15 mL polypropylene centrifuge tubes and the suspension was vortexed for 20 seconds and allowed to rest on ice for an additional 10 minutes. The extract was centrifuged at 5000 x g for 10 minutes, and the supernatant was divided into 1.5 mL aliquots which were completely dried under a gentle stream of nitrogen gas. Dried samples were reconstituted in varied volumes of 90:10 water:methanol (for RPLC analyses) and 85:15 methanol:water (for HILIC). |
| Sampleprep Protocol Filename: | Mod_vs_Con_Chrom_IDs_Protocol.pdf |
| Processing Storage Conditions: | On ice |
| Extraction Method: | Solvent precipitation with 4 parts 1:1:1 methanol:acetonitrile:acetone to 1 part human plasma |
| Extract Cleanup: | Centrifugation 5000 g for 10 min |
| Extract Storage: | 4℃ |
| Sample Resuspension: | 90:10 water:methanol (RPLC)/85:15 methanol:water (HILIC) volume varied |
