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MB Sample ID: SA188751
| Local Sample ID: | Pat_76_NEG |
| Subject ID: | SU002097 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
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Sample Preparation:
| Sampleprep ID: | SP002103 |
| Sampleprep Summary: | plasma samples were thawed on ice and 50 µL of plasma was retained for the metabolome analysis. Approximately 200 µL of cold methanol was added to the plasma sample and shaken vigorously to inactivate any potential viruses. The samples were stored in -80 ºC immediately until extraction for metabolomics experiments. For extraction, 200 µL of cold methanol was added to each tube, vortexed and shaken vigorously for 30 min at 4 ºC in a temperature controlled thermal shaker. Further 200 µL of chloroform and 400 µL of water were added, shaken vigorously and the top aqueous phase was recovered as a metabolite mixture of diverse chemical nature. Each metabolite extract was dried overnight in speedVac and reconstituted in 60 µL of 0.1% formic acid in water. Ten microliters from each tube were removed to create a pooled QC sample that was injected at the beginning and end of the MS sequence run and additional QC samples were injected after every 10 sample injections. |
| Processing Storage Conditions: | Described in summary |
| Extraction Method: | Described above |
| Extract Storage: | Described in summary |
| Sample Resuspension: | 0.1% formic acid |
