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MB Sample ID: SA189528

Local Sample ID:	S-A116-G12-M
Subject ID:	SU002105
Subject Type:	Mammal
Subject Species:	Rattus norvegicus
Taxonomy ID:	10116

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Sample Preparation:

Sampleprep ID:	SP002111
Sampleprep Summary:	Tissue metabolites were extracted according to our laboratory standard protocol. Briefly, about 100 mg liver and all received thyroid gland (2.4 - 34.1 mg) tissue materials were weighed and mixed with 5x extraction buffer (ACN / H2O: 1:1). With the help of added 4 metal balls, the tissue metabolites were separated by using a tissue slicer for 10 min at 30 Hz. After centrifugation of 2 min at 14,000 rpm, the metabolites containing supernatant were transfered to a fresh tube, and stored at -80℃ for further analysis. The targeted metabolomics was done by using MxP® Quant 500 kit (Biocrates). All analysis steps was followed by the manufactures from Biocrates.

Sampleprep ID:

SP002111

Sampleprep Summary:

Tissue metabolites were extracted according to our laboratory standard protocol. Briefly, about 100 mg liver and all received thyroid gland (2.4 - 34.1 mg) tissue materials were weighed and mixed with 5x extraction buffer (ACN / H2O: 1:1). With the help of added 4 metal balls, the tissue metabolites were separated by using a tissue slicer for 10 min at 30 Hz. After centrifugation of 2 min at 14,000 rpm, the metabolites containing supernatant were transfered to a fresh tube, and stored at -80℃ for further analysis. The targeted metabolomics was done by using MxP® Quant 500 kit (Biocrates). All analysis steps was followed by the manufactures from Biocrates.