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MB Sample ID: SA200490

Local Sample ID:	16382Y
Subject ID:	SU002173
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Age Or Age Range:	40-80
Gender:	Male and female
Human Race:	Non-hispanic White and African-American
Human Smoking Status:	Current/Former/Never

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Sample Preparation:

Sampleprep ID:	SP002179
Sampleprep Summary:	P100 plasma was profiled using the Metabolon (Durham, USA) Global Metabolomics Platform, as described (2-4). Briefly, samples were extracted with methanol under vigorous shaking for two minutes (Glen Mills GenoGrinder 2000) followed by centrifugation to remove protein, dissociate small molecules bound to protein or trapped in the precipitated protein matrix, and to recover chemically diverse metabolites. The resulting extract was divided into five fractions: two for analysis by two separate reverse phase/ultrahigh performance liquid chromatography/tandem mass spectrometry (RP/UPLC-MS/MS) methods with positive ion mode electrospray ionization (ESI), one for analysis by RP/UPLC-MS/MS with negative ion mode ESI, one for analysis by hydrophilic interaction chromatography (HILIC)/UPLC-MS/MS with negative ion mode ESI, and one sample was reserved for backup.

Sampleprep ID:

SP002179

Sampleprep Summary:

P100 plasma was profiled using the Metabolon (Durham, USA) Global Metabolomics Platform, as described (2-4). Briefly, samples were extracted with methanol under vigorous shaking for two minutes (Glen Mills GenoGrinder 2000) followed by centrifugation to remove protein, dissociate small molecules bound to protein or trapped in the precipitated protein matrix, and to recover chemically diverse metabolites. The resulting extract was divided into five fractions: two for analysis by two separate reverse phase/ultrahigh performance liquid chromatography/tandem mass spectrometry (RP/UPLC-MS/MS) methods with positive ion mode electrospray ionization (ESI), one for analysis by RP/UPLC-MS/MS with negative ion mode ESI, one for analysis by hydrophilic interaction chromatography (HILIC)/UPLC-MS/MS with negative ion mode ESI, and one sample was reserved for backup.