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MB Sample ID: SA200937
| Local Sample ID: | HF-L-4RL |
| Subject ID: | SU002177 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | 21 weeks |
| Gender: | Male |
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Sample Preparation:
| Sampleprep ID: | SP002183 |
| Sampleprep Summary: | Mouse lungs were weighed into Eppendorf tubes. The lung tissues were mashed using a clean metal spatula prior to extraction. The samples were extracted using a liquid-liquid partition with water (250 µL), methanol (300 µL), and methyl tert-butyl ether (MTBE). Avanti’s deuterated lipid mix, Equisplash, was used as an internal standard. The deuterated mix was spiked into the methanol at 1.5 µg/mL and used for extraction. The extracts were centrifuged at 20,000 rcf for 10 minutes to facilitate phase separation. The top layer was removed, dried, and reconstituted in 150 µL of isopropanol for analysis. |
| Processing Storage Conditions: | -80℃ |
| Sample Resuspension: | 150 µL of IPA |
