Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA204237

Local Sample ID:	M-4
Subject ID:	SU002206
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

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Sample Preparation
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MS

Sample Preparation:

Sampleprep ID:	SP002212
Sampleprep Summary:	100-130 mg tissue samples tumor tissues have to be broken by oscillation with 60HZ and 2 minutes in iced 80% methanol solution (mixed with internal standard 0.001mg/ml 4-chloro-DL-phenylalanine). centrifuging at 20,000 g for 10 min at 4 °C, and mixing the supernatants with 800 μL ice-cold acetonitrile. Then the supernatant obtained by centrifugation was filtered through a 0.22 µm organic phase membrane, blown dry in nitrogen, reconstituted in 100 µL of water and transferred to vials for metabolomics assay.

Sampleprep ID:

SP002212

Sampleprep Summary:

100-130 mg tissue samples tumor tissues have to be broken by oscillation with 60HZ and 2 minutes in iced 80% methanol solution (mixed with internal standard 0.001mg/ml 4-chloro-DL-phenylalanine). centrifuging at 20,000 g for 10 min at 4 °C, and mixing the supernatants with 800 μL ice-cold acetonitrile. Then the supernatant obtained by centrifugation was filtered through a 0.22 µm organic phase membrane, blown dry in nitrogen, reconstituted in 100 µL of water and transferred to vials for metabolomics assay.