Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA204748

Local Sample ID:	11887
Subject ID:	SU002217
Subject Type:	Mammal
Subject Species:	Papio hamadryas
Taxonomy ID:	9557

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Sample Preparation:

Sampleprep ID:	SP002223
Sampleprep Summary:	15 μL of plasma or liver samples were subjected to sequential solvent extraction, once each with 1 mL of acetonitrile: isopropanol: water (3:3:2) and 500 μL of acetonitrile: water (1:1) mixtures at 4°C [14]. An internal standard, adonitol (2 μL from 10 mg/ml stock) was added to each aliquot prior to the extraction. The extracts were dried under vacuum at 4°C prior to chemical derivatization (silylation reactions). Blank tubes without samples, were treated similarly as sample tubes and added to account for background noise and other sources of contamination. Samples and blanks were sequentially derivatized with meth-oxyamine hydrochloride (MeOX) and 1% TMCS in N-methyl-N-trimethylsilyl-trifluoroacetamide (MSTFA) or 1% TMCS containing N-(t-butyldimethylsilyl)-N-methyltrifluoroacetamide (MTBSTFA) as described elsewhere [15]. Briefly, the steps involved addition of 20 μL of MeOX (20 mg mL-1) in pyridine incu-bated at 55°C for 60 min followed by trimethylsilylation at 60°C for 60 min after adding 80 μL MTBSTFA.

Sampleprep ID:

SP002223

Sampleprep Summary:

15 μL of plasma or liver samples were subjected to sequential solvent extraction, once each with 1 mL of acetonitrile: isopropanol: water (3:3:2) and 500 μL of acetonitrile: water (1:1) mixtures at 4°C [14]. An internal standard, adonitol (2 μL from 10 mg/ml stock) was added to each aliquot prior to the extraction. The extracts were dried under vacuum at 4°C prior to chemical derivatization (silylation reactions). Blank tubes without samples, were treated similarly as sample tubes and added to account for background noise and other sources of contamination. Samples and blanks were sequentially derivatized with meth-oxyamine hydrochloride (MeOX) and 1% TMCS in N-methyl-N-trimethylsilyl-trifluoroacetamide (MSTFA) or 1% TMCS containing N-(t-butyldimethylsilyl)-N-methyltrifluoroacetamide (MTBSTFA) as described elsewhere [15]. Briefly, the steps involved addition of 20 μL of MeOX (20 mg mL-1) in pyridine incu-bated at 55°C for 60 min followed by trimethylsilylation at 60°C for 60 min after adding 80 μL MTBSTFA.