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MB Sample ID: SA207563
Local Sample ID: | DV3 |
Subject ID: | SU002250 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
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Sample Preparation:
Sampleprep ID: | SP002256 |
Sampleprep Summary: | 1x108 293FT, TMEM41B KO and VMP1 KO cells were washed with PBS thrice before adding 560 µl extraction solvent (methanol:water = 2:5), pre-cooled at −4°C. Cells were then scraped into the extraction solvent on ice and transferred into eppendorf tubes. Then, 800 µL methyl tert-butyl ether (MTBE) was added and the samples were sonicated for 10 min. After centrifugation for 15 min at 3,000 rpm at 4°C to separate phases, the upper layer and lower layer were collected for lipidomics and metabolomics analysis, respectively. |