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MB Sample ID: SA217741
| Local Sample ID: | DHEA3 |
| Subject ID: | SU002356 |
| Subject Type: | Other organism |
| Subject Species: | Xenopus tropicalis |
| Age Or Age Range: | Stage 41-43 |
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Sample Preparation:
| Sampleprep ID: | SP002362 |
| Sampleprep Summary: | Aqueous metabolites for targeted LC-MS analysis were extracted using a protein precipitation method similar to the one described elsewhere (Mathon et al., 2019; Meador et al., 2020). Samples were first homogenized in 200 µL purified deionized water at 4 ˚C, and then 800 µL of methanol containing 6C13-glucose and 2C13-glutamate (reference internal standards) was added. Afterwards samples were vortexed, stored for 30 minutes at -20 ˚C, sonicated in an ice bath for 10 minutes, centrifuged for 15 min at 14,000 rpm and 4 ˚C, and then 600 µL of supernatant was collected from each sample. Lastly, recovered supernatants were dried on a SpeedVac and reconstituted in 1.0 mL of LC-matching solvent containing 2C13-tyrosine and 3C13-lactate (reference internal standards). |
| Sampleprep Protocol Filename: | JPatel_LC-MS_Methods.pdf |
