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MB Sample ID: SA232175

Local Sample ID:	plasma # 227
Subject ID:	SU002424
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

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Sample Preparation:

Sampleprep ID:	SP002430
Sampleprep Summary:	To capture a broad spectrum of metabolites, we used the MxP® Quant 500 kit combined with the in-house acylcarnitine (AC) assay (biocrates life sciences ag). Samples were prepared the same way for both assays. Therefore, 50-80 mg of tumor tissues were transferred to 0.5 ml Precellys® vials followed by the addition of 85:15 ethanol:0.01 M phosphate as a lysis buffer with a volume [µl] of 3 times the tissue weight [mg]. Tissue homogenization was carried out at 4 °C and 5800 rpm using a Precellys® 24 homogenizer coupled to a Cryolys® cooling unit. The homogenates were centrifuged at 10,000 g for 2 min at 2-4 °C. Supernatants were collected and stored at -80 °C until analysis. Plasma samples as well as tissue homogenate supernatant were thawed on ice, then vortexed before use.

Sampleprep ID:

SP002430

Sampleprep Summary:

To capture a broad spectrum of metabolites, we used the MxP® Quant 500 kit combined with the in-house acylcarnitine (AC) assay (biocrates life sciences ag). Samples were prepared the same way for both assays. Therefore, 50-80 mg of tumor tissues were transferred to 0.5 ml Precellys® vials followed by the addition of 85:15 ethanol:0.01 M phosphate as a lysis buffer with a volume [µl] of 3 times the tissue weight [mg]. Tissue homogenization was carried out at 4 °C and 5800 rpm using a Precellys® 24 homogenizer coupled to a Cryolys® cooling unit. The homogenates were centrifuged at 10,000 g for 2 min at 2-4 °C. Supernatants were collected and stored at -80 °C until analysis. Plasma samples as well as tissue homogenate supernatant were thawed on ice, then vortexed before use.