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MB Sample ID: SA239083
Local Sample ID: | QC_Medium_full-MS-2-1 |
Subject ID: | SU002488 |
Subject Type: | Cultured cells |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Subject Comments: | OP9 cells were left as stromal cells or were differentiated into adipocytes. These cells were allowed to condition media, with the media being the analyte in this study. |
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Sample Preparation:
Sampleprep ID: | SP002494 |
Sampleprep Summary: | Media samples (50 µL) were mixed with acetonitrile (Sigma) (100 µL) and vortexed. All samples were incubated 30 min on ice and centrifuged at 20,627 g for 10 min at 4 ˚C. The supernatant was transferred to autosampler vials (ThermoFisher) held at 4 ˚C for LC-MS acquisition. |
Processing Storage Conditions: | On ice |