Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA240909

Local Sample ID:	1458
Subject ID:	SU002496
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606

Select appropriate tab below to view additional metadata details:

Sample Preparation:

Sampleprep ID:	SP002502
Sampleprep Summary:	For all non-targeted analyses, 10 µL of intestinal lumen samples were subjected to a modified biphasic water, methanol, and methyl tert-butyl ether extraction 78 to separate polar and non-polar metabolites. The polar and non-polar phases were divided into multiple aliquots in 96-well plates, dried by rotary vacuum, and frozen until further analysis. Homogenized stool samples were prepared using an analogous extraction procedure with modification for bead-homogenization and extraction in microcentrifuge tubes to account for the solid nature of the sample. Targeted bile acid analysis was performed using aqueous phase of the described biphasic extraction. Targeted SCFA analysis used an acidified water and MTBE extraction followed by MTBSTFA derivatization 79 . For detailed sample preparation methods see Supplemental Material.

Sampleprep ID:

SP002502

Sampleprep Summary:

For all non-targeted analyses, 10 µL of intestinal lumen samples were subjected to a modified biphasic water, methanol, and methyl tert-butyl ether extraction 78 to separate polar and non-polar metabolites. The polar and non-polar phases were divided into multiple aliquots in 96-well plates, dried by rotary vacuum, and frozen until further analysis. Homogenized stool samples were prepared using an analogous extraction procedure with modification for bead-homogenization and extraction in microcentrifuge tubes to account for the solid nature of the sample. Targeted bile acid analysis was performed using aqueous phase of the described biphasic extraction. Targeted SCFA analysis used an acidified water and MTBE extraction followed by MTBSTFA derivatization 79 . For detailed sample preparation methods see Supplemental Material.