Return to study ST002422 main page
MB Sample ID: SA242403
Local Sample ID: | 20220508_pos_WT_rep1_16ul |
Subject ID: | SU002511 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Cell Strain Details: | HEK293T |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP002517 |
Sampleprep Summary: | Lipids were isolated from collected cultured cells. Cells were washed with PBS, treated with cold 50% methanol (1mL) and transferred to glass vials. Next, chloroform (0.5mL) was added and samples were gently vortexed and centrifuged at 1,000x g for 5 min at 4˚C. Lipids were transferred to a clean glass vial using a glass Hamilton syringe. Lipids were extracted twice using chloroform prior to being dried under nitrogen gas. Samples were normalized according to protein concentration when resuspended in a 1:1:1 solution of methanol:chloroform:isopropanol prior to mass spectrometry (MS) analysis. To assist quantification various volumes were injected for MS (4, 8, and 16ul for positive mode analysis and 5, 10, and 15ul for negative mode analysis). |