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MB Sample ID: SA246367
Local Sample ID: | HR3 |
Subject ID: | SU002551 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
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Sample Preparation:
Sampleprep ID: | SP002557 |
Sampleprep Summary: | Samples were thawed and add with 800μL of 80% methanol, vortexed for 30 s. Then all samples were extracted at 4°C with ultrasound for 30 mins, kept at -40°C for 1 h, and centrifuged at 12000 rpm and 4°C for 15 mins. Finally, supernatant dried down under a nitrogen gas, the residue was resuspended with 100μL of 80% methanol and 5μL of DL-o-Chlorophenylalanine (140μg/mL) for LC-MS analysis. |