Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA246839

Local Sample ID:	liv_sdm_3_F_1
Subject ID:	SU002555
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090
Age Or Age Range:	3-12 weeks
Animal Animal Supplier:	In house breeding
Animal Housing:	Germ-free and gnotobiotic isolators for germ and gnotobiotic mice. Individually ventilated cages for specific pathogen free mice
Animal Light Cycle:	12 hours
Animal Feed:	LabDiet (Cat# 5010; Laboratory Autoclavable Rodent Diet)

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Sample Preparation:

Sampleprep ID:	SP002561
Sampleprep Summary:	Mice were anaesthetized with isoflurane and blood was collected by retro-orbital bleed to serum separation collection tubes (BD, NJ, USA). Urine was collected from the mouse either before it was euthanized, immediately following euthanization or directly from the bladder following euthanization. Mice were euthanized by cervical dislocation. 1.5mL of ice-cold sterile PBS was injected into the peritoneal cavity, massaged for 20 seconds then removed and collected. The spleen and a segment of left lobe of the liver was collected. The intestine was excised and content from the entire length of the jejunum, ileum, cecum, and colon were collected. For the purposes of these experiments, the first 10cm of the small intestine was considered duodenum, and the remaining length was split in half with the proximal segment considered jejunum and the distal segment considered ileum. Following collection, blood was centrifuged (10,000 xg, 10 minutes, 4°C) and serum transferred into a 1.5mL Eppendorf tube. All collected samples were placed in liquid nitrogen immediately after collection and stored at -80°C until processing.

Sampleprep ID:

SP002561

Sampleprep Summary:

Mice were anaesthetized with isoflurane and blood was collected by retro-orbital bleed to serum separation collection tubes (BD, NJ, USA). Urine was collected from the mouse either before it was euthanized, immediately following euthanization or directly from the bladder following euthanization. Mice were euthanized by cervical dislocation. 1.5mL of ice-cold sterile PBS was injected into the peritoneal cavity, massaged for 20 seconds then removed and collected. The spleen and a segment of left lobe of the liver was collected. The intestine was excised and content from the entire length of the jejunum, ileum, cecum, and colon were collected. For the purposes of these experiments, the first 10cm of the small intestine was considered duodenum, and the remaining length was split in half with the proximal segment considered jejunum and the distal segment considered ileum. Following collection, blood was centrifuged (10,000 xg, 10 minutes, 4°C) and serum transferred into a 1.5mL Eppendorf tube. All collected samples were placed in liquid nitrogen immediately after collection and stored at -80°C until processing.