Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA247974

Local Sample ID:	PMN_90
Subject ID:	SU002567
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Sample Preparation
Chromatography
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MS

Sample Preparation:

Sampleprep ID:	SP002573
Sampleprep Summary:	Metabolites from pellets containing 2 million neutrophils were extracted with 100 μl of chilled solvent of 40:40:20 methanol:acetonitrile:water (v:v:v) per million cells. The cell pellet was resuspended in the extraction solvent, vortexed for 30’’, and freeze-thawed 3 times. Then the samples were centrifuged at 21,000 g for 15 minutes at 4 °C and the supernatant transferred to LC-MS autosampler vials.

Sampleprep ID:

SP002573

Sampleprep Summary:

Metabolites from pellets containing 2 million neutrophils were extracted with 100 μl of chilled solvent of 40:40:20 methanol:acetonitrile:water (v:v:v) per million cells. The cell pellet was resuspended in the extraction solvent, vortexed for 30’’, and freeze-thawed 3 times. Then the samples were centrifuged at 21,000 g for 15 minutes at 4 °C and the supernatant transferred to LC-MS autosampler vials.