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MB Sample ID: SA001768

Local Sample ID:Test_1P
Subject ID:SU000057
Subject Type:Invertebrate
Subject Species:Caenorhabditis elegans
Taxonomy ID:6239
Genotype Strain:N2
Age Or Age Range:Young Adult
Gender:Hermaphrodite
Species Group:Invertebrate

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Treatment:

Treatment ID:TR000058
Treatment Summary:Heat Shock: Two successive synchronous generations of wild-type C. elegans (N2) were grown in S-complete buffer at 22C containing 5% 13C-labeled E. coli. Upon reaching the young adult stage, the population was washed from E. coli using standard procedures and was divided into four replicates. Each replicate was treated with a 30 min heat shock at 33 °C followed by incubation at 22C for 1.5 hours. Control: Two successive synchronous generations of wild-type C. elegans (N2) were grown in S-complete buffer at 22C containing 95% 13C-labeled E. coli. Upon reaching the young adult stage, the population was washed from E. coli using standard procedures.
Treatment:Abiotic
Treatment Dose:30C | 22C
Treatment Doseduration:30C for 30 minutes; 1.5 hours at 22 C before harvest | 22C for 30 minutes; 1.5 hours at 22 C before harvest
Animal Acclimation Duration:-
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