Return to study ST000055 main page
MB Sample ID: SA002838
| Local Sample ID: | MR2_CTL_197 |
| Subject ID: | SU000074 |
| Subject Type: | Animal cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | FLT3-ITD AML (MR2) |
| Cell Biosource Or Supplier: | Dr. Tim Pardee (Wake Forest Univ.) |
| Cell Primary Immortalized: | Primary |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Treatment:
| Treatment ID: | TR000075 |
| Treatment Summary: | MEK kinase inhibitors |
| Treatment Compound: | GSK | AZD |
| Treatment Route: | added to media |
| Treatment Dose: | 10nM | 10nM |
| Treatment Vehicle: | dilutions made in 1X sterile PBS (orginally dissolved in DMSO) |
| Cell Storage: | liquid nitrogen, live maintenance in 5% CO2, humidified incubator |
| Cell Growth Container: | T-75 culture flask |
| Cell Growth Config: | suspension |
| Cell Growth Rate: | double ~every 20 hours |
| Cell Media: | 50:50 DMEM:IMDM + 10% FBS, 1% P/S |
| Cell Harvesting: | Transfer 1 mL to new flask containing 10 mL of fresh culture media |
| Cell Pct Confluence: | maintained at ~ 2 x10^6/mL |
