Return to study ST000084 main page

MB Sample ID: SA004283

Local Sample ID:SBEP_Metab_LPSactivated2
Subject ID:SU000103
Subject Type:Animal cells
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:RAW 264.7
Cell Strain Details:RAW 264.7
Cell Primary Immortalized:immortalized
Species Group:Mammal

Select appropriate tab below to view additional metadata details:

Treatment:

Treatment ID:TR000104
Treatment Summary:Macrophages (RAW 264.7 cells) grown for two day with suplimentation of 100ng | ml lipopolysaccharide | Macrophages (RAW 264.7 cells) grown for two days without suplimentation
Treatment Protocol Comments:Macrophages (RAW 264.7 cells) were seeded at a density of 3.0E6 cells in 150 mm dishes using Dulbecco's Modified Eagle's Medium supplemented with 10% fetal calf serum, then grown for 2 days and stimulated for 24 hours with 100 ng/mL of lipopolysaccharide diluted in fresh medium. A control culture was run in parallel by incubating for the same period of time with fresh medium only. Three biological replicates were performed per condition, and two dishes were used for each replicate. / Macrophages (RAW 264.7 cells) were seeded at a density of 3.0E6 cells in 150 mm dishes using Dulbecco's Modified Eagle's Medium supplemented with 10% fetal calf serum, then grown for 2 days and stimulated for 24 hours with 100 ng/mL of lipopolysaccharide diluted in fresh medium. A control culture was run in parallel by incubating for the same period of time with fresh medium only. Three biological replicates were performed per condition, and two dishes were used for each replicate.
Treatment:Abiotic
Treatment Compound:lipopolysaccharide / fresh medium
Treatment Dose:100 ng/mL /--
Treatment Vehicle:fresh medium
Cell Growth Container:150 mm dishes
Cell Inoc Proc:Macrophages (RAW 264.7 cells) were seeded at a density of 3.0E6 cells in 150 mm dishes
Cell Media:Dulbecco's Modified Eagle's Medium supplemented with 10% fetal calf serum
Cell Harvesting:After stimulation, cells were washed twice with Dulbecco’s PBS, scraped out and harvested into 15-mL centrifuge tubes.
  logo