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MB Sample ID: SA017089
| Local Sample ID: | 110624baasa15_1 |
| Subject ID: | SU000400 |
| Subject Type: | Cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
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Treatment:
| Treatment ID: | TR000414 |
| Treatment Summary: | 3 Treatments: A) Control group of HepG2 cells incubated in media with 5.5 mM glucose (Glc5) B) HepG2 cells incubated in media containing 5.5 mM glucose + 5.0 mM fructose (Glc5Fru5) C) HepG2 cells incubated in media containing 10.5 mM glucose (Glc10) |
| Treatment Protocol Filename: | metabolomic_responses_of_cultured_HepG2_liver_cells.pdf |
| Treatment Protocol Comments: | HepG2 cells incubated in media containing 10.5 mM glucose (Glc10) was included to enable differentiation of metabolic effects caused by fructose from metabolic effects caused by increased hexose resources |
| Cell Media: | Cells were acclimated to their respective media condition for 48 h prior to collection of sample material to obtain a metabolite profile representative of continued exposure instead of response to a sudden change in carbohydrate resources. Media was replenished after 24 and 48 h |
