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MB Sample ID: SA018731
| Local Sample ID: | 061215byusa1067_1 |
| Subject ID: | SU000414 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57Bl/6J |
| Age Or Age Range: | 4 months |
| Gender: | Female |
| Human Smoking Status: | Former/Current |
| Animal Animal Supplier: | Jackson Laboratories |
| Animal Housing: | housed in a facility at 23°C |
| Animal Light Cycle: | 12∶12 hr light cycle, lights on 0700 |
| Animal Feed: | with free access to chow (4.5% fat/weight), for at least 1 week before being studied |
| Species Group: | Mammal |
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Treatment:
| Treatment ID: | TR000428 |
| Treatment Summary: | Mitochondria (0.6 mg/ml) were supplied with three concentrations of palmitate corresponding to rates of β-oxidation: 1. low (2 µM) 2. medium (9 µM) 3. high (19 µM) Three ml aliquots of incubation medium [IM, (mM): KCl (120), HEPES (5), KH2PO4 (5), MgCl2 (5) and EGTA (1); pH 7.4] were supplemented with (mM) ATP (1), malate (0.05), coenzyme A (0.025), and carnitine (0.5) and added to 20-ml glass reaction vials. Solutions of low, medium and high palmitate concentrations were added to vials in a 6∶1 FA:BSA complex. Two additional incubations were performed as controls: 1. 0 µM palmitate 2. 9 µM palmitate + inhibitors |
| Treatment Protocol Filename: | Long-Chain_Fatty_Acid_Metabolite_Profiles_in_Skeletal_Muscle_Mitochondria.PDF |
| Treatment Protocol Comments: | The first control condition evaluated the metabolic profile of mitochondria oxidizing only malate, and included ATP, carnitine and CoA and ethanol (0.5%). The second control condition assessed effects of FA in the absence of complete oxidative catabolism, and consisted of malate, 9 µM palmitate, ATP, carnitine and CoA, and supplemented with the TCA cycle inhibitor malonate (10 mM) and the electron transport chain complex I inhibitor rotenone (5 µM) |
