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MB Sample ID: SA020509
| Local Sample ID: | ciMB1_D02c |
| Subject ID: | SU000435 |
| Subject Type: | Cells |
| Subject Species: | Plasmodium falciparum |
| Taxonomy ID: | 5833 |
| Genotype Strain: | 3D7 |
| Species Group: | Microorganism |
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Treatment:
| Treatment ID: | TR000449 |
| Treatment Summary: | Cultures (200 µL) were incubated with test compounds (1 µM) for 5 hours in 96-well plates. Four replicate incubations of each compound were conducted and analyzed. Untreated controls contained equivalent amounts of DMSO (as vehicle), and additional ‘quench test’ controls were prepared whereby the test compounds were added after the quenching step, to allow detection of test compound-derived LC-MS features that did not arise from biochemical metabolism within the cells. Each plate contained 10 or 20 test compounds in addition to positive controls (artemisinin and/or atovaquone) and negative controls (untreated DMSO and ‘quench test’ described above). Incubations and extractions for the 100 test compounds (10 compounds with known antimalarial activity and 90 of the highest priority compounds from the Malaria Box including all of plate A and the first row of plate B) were performed in three separate batches, with the known antimalarial compounds and first 10 Malaria Box compounds in the first batch, the next 40 Malaria Box compounds in the second batch, and the following 40 Malaria Box compounds in the third batch. |
| Treatment Compound: | Antimalarials and MMV Malaria Box compounds |
| Treatment Dose: | 1 micromolar |
| Treatment Doseduration: | 5 hours |
| Treatment Vehicle: | DMSO |
| Cell Growth Container: | 96-well V-bottomed plate |
| Cell Media: | RPMI-HEPES |
| Cell Envir Cond: | 37 °C under defined atmosphere (95% N2, 4% CO2, 1% O2) |
| Cell Media Lastchanged: | 18 hours |
