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MB Sample ID: SA028305
| Local Sample ID: | HR_10_IDH1 |
| Subject ID: | SU000570 |
| Subject Type: | Cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
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Treatment:
| Treatment ID: | TR000584 |
| Treatment Summary: | 239T cells (ATCC CRL-3216) were grown in DMEM (Invitrogen) with 10% FBS (Hyclone) at -37°C in 10% CO2 and transfected with pcDNA3.1, pcDNA3.1-IDH2WT, pcDNA3.1-IDH2R172K (Invitrogen and Origene) with Lipofectamine 2000 (Invitrogen) according to manufacturer instructions. 6x10^5 cells were seeded in 6 well plates for protocol 1 and 3.5x10^6 cells were seeded in 10 cm plates for protocol 2. Identity of all vectors was confirmed by sequencing and vector integrity with agarose gel electrophoresis.The common feature of IDH1 and IDH2 mutations.pdf |
| Treatment Protocol Filename: | 239T cells (ATCC CRL-3216) were grown in DMEM (Invitrogen) with 10% FBS (Hyclone) at 37°C in 10% CO2 and transfected with pcDNA3.1, pcDNA3.1-IDH2WT, pcDNA3.1-IDH2R172K (Invitrogen and Origene) with Lipofectamine 2000 (Invitrogen) according to manufacturer instructions. |
| Cell Harvesting: | 24-48 hours after transfection |
