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MB Sample ID: SA028929
| Local Sample ID: | 236 |
| Subject ID: | SU000583 |
| Subject Type: | Plant |
| Subject Species: | Solanum lycopersicum |
| Taxonomy ID: | 4081 |
| Genotype Strain: | S. lycopersicum X S. pimpinellifolium |
| Age Or Age Range: | Dry Seeds - 6 hours Imbibed Seeds |
| Species Group: | Plant |
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Treatment:
| Treatment ID: | TR000597 |
| Treatment Summary: | In this study the F8 population of 100 Recombinant Inbred Lines (RILs) obtained from a cross between Solanum lycopersicum X Solanum pimpinellifolium were intelligently allocated to two sub-populations optimized for the distribution of parental alleles using the R-procedure DesignGG (Li et al., 2009; Joosen et al., 2013); hence 50 RIL lines were used for dry seeds and 50 lines for 6h imbibed seeds |
| Treatment: | Metabolites were extracted from dry and 6 hour imbibed seeds |
| Treatment Route: | Seed |
| Plant Growth Location: | The S. lycopersicum × S. pimpinellifolium RIL population was grown twice under controlled conditions in the greenhouse facilities at Wageningen University, in the Netherlands. |
| Plant Plot Design: | RCBD |
| Plant Light Period: | 16h light and 8h dark (long-day conditions) |
| Plant Humidity: | 30% RH |
| Plant Temp: | The day and night temperatures were maintained at 25 °C and 15 °C, respectively |
| Plant Watering Regime: | Watered Daily |
| Plant Nutritional Regime: | All the RILs were uniformly supplied with the basic dose of fertilizer |
| Plant Growth Stage: | Seeds were extracted from healthy fruits and treated with 1% hydrochloric acid (HCL) to remove the large pieces of the pulp that were sticking onto the seeds. |
| Plant Storage: | The cleaned seeds were dried for 3d at 20 °C and were stored in a cool, dry storage room (13 °C and 30% RH) in paper bags |
