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MB Sample ID: SA060913
| Local Sample ID: | GLA_QEx_Lipids_SO119_SA121_1007dG2 |
| Subject ID: | SU001027 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
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Treatment:
| Treatment ID: | TR001041 |
| Treatment Summary: | For this study, we used a subset of samples from our recent study focused on nutritional phenotyping in response to a test meal containing gamma-linolenic acid. Briefly, in a single blind, placebo-controlled, crossover design, seven healthy subjects consumed a test meal that consisted of GLA fat (borage oil [denoted GF]) or a control fat (a mixture of corn, safflower, sunflower, and extra-virgin light olive oils [denoted CF]). Compared to the original study, where all subjects were fed on three separate test days for each test meal, a small modification was needed due to sample limitation. Thus, for this study, six subjects were fed on three separate test days for each test meal, while one subject was fed on two separate test days for a control fat meal and four test days for GLA fat (the fourth set was not used in the original study). Plasma samples collected at 0, 2, and 4h in response to the test meals were used for analysis. In total, 126 samples were analyzed out of which 42 were baseline samples (time 0 h), 40 were control fat samples (time 2 and 4 h), and 44 were GLA fat samples (time 2 and 4 h). For quality control, a pool sample consisted of a mixture of nonfasting blood plasma (both control and GLA fat) was used. Also, standard reference material SRM 1950 Metabolites in Frozen Human Plasma (NIST, Gaithersburg, MD) was used. |
| Treatment Protocol Filename: | acs_analchem_7b03404.pdf |
