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MB Sample ID: SA063977
Local Sample ID: | #B10_0095 YKT_11/8/16_#83 |
Subject ID: | SU001058 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | FVB/N |
Age Or Age Range: | 16.5 to 18.5 weeks old |
Weight Or Weight Range: | 25 to 30 grams |
Gender: | Male |
Animal Animal Supplier: | in house breeding |
Animal Feed: | Standard chow |
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Treatment:
Treatment ID: | TR001072 |
Treatment Summary: | Swim training - Physiological hypertrophy in mice was achieved via swim training twice daily for 4 weeks, as previously described (McMullen et al., 2003). Swim training commenced in adult mice at approximately 3 months of age with 10 min sessions on the first day, with 10 min increments each day until the maximum of 90 min per session was reached. Mice were rested for at least 4 hours between each session, and water temperature was maintained between 30 to 32°C to avoid thermal stress. At the end of every session, mice were individually towel dried to prevent hypothermia. Plasma and hearts were collected 3 h after the last swim session. Transverse aortic constriction - Male mice were subjected to either sham or TAC surgery at approximately 3-3.5 months of age, as previously described (Xu et al., 2008). Two variations of TAC were performed, designated TAC-moderate (TAC-MOD) and TAC-severe (TAC-SEV). Aortic banding performed on the TAC-MOD mice used a blunt 25 G needle (outside diameter 0.5 mm) placed next to the aorta during ligation to obtain a consistent degree of banding (constriction of blood vessel from ~1 mm to 0.5 mm), while a 27 G needle (outside diameter 0.4 mm) was used for the TAC-SEV group (constriction of blood vessel from ~1 mm to 0.4 mm). Sham operated mice were subjected to the same procedure but no ligation was made. |