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MB Sample ID: SA072459
| Local Sample ID: | HA_22 |
| Subject ID: | SU001117 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | Tcf/LacZ mice |
| Age Or Age Range: | 6-8 weeks |
| Gender: | Male and female |
| Animal Inclusion Criteria: | The Tcf/LacZ strain was previously backcrossed onto the C3H background C3H background, and confirmed by PCR to lack the rd1 mutation |
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Treatment:
| Treatment ID: | TR001131 |
| Treatment Summary: | Mice of either sex were randomly selected to receive intravitreal injection of recombinant 20ng Wnt3a ligand group or saline control group (saline was injected with equivalent volume as recombinant 20ng Wnt3a ligand). A small diabetic needle was used to make an incision in the superior posterior area of the conjunctiva-sclera border of the left eye. After which, either recombinant 20ngWnt3a ligand or saline was injected intravitreally using a 1.5 cm 33-gauge Hamilton needle (Hamilton Company, Reno, NV). The injection needle was angled to avoid hitting the lens. The surface membrane of the left eye was cut around the conjunctiva-sclera border. Dumont #5 forceps were inserted between the membrane and the globe to move the surrounding tissues while searching for the optic nerve. Once the optic nerve was located, the forceps’ teeth surrounded the nerve 1 mm from the globe and crushed the nerve for 5 seconds. The crush is successful if there is very minimal or no damage to the surrounding blood supply. Affected left eyes were treated with topical erythromycin ointment and 1mg/ml of Buprenorphine-SR lab was injected subcutaneously. Animal Anesthesia: ketamine/xylazine cocktail intraperitoneally, eyes were locally anesthetized with 0.5% proparacaine hydrochloride.Any mouse with excessive bleeding around the eye was excluded from the study. Mouse’s overall health and left eye’s health were monitored daily from day 1 post crush until day 7 post crush. Animal Endpoint Clinical Signs: Mice portraying lethargy 24 hours after surgery or eye infection to the affected eye days after surgery were excluded from the study. |
| Treatment: | optic nerve crush, intravitreal injection |
| Treatment Compound: | Wnt3a or saline |
| Treatment Route: | intravitreal injection |
| Treatment Dosevolume: | 20 ng of Wnt3a or equal volume of saline |
| Treatment Doseduration: | single injection |
| Animal Anesthesia: | See summary |
| Animal Endp Euthanasia: | CO2 + decapitation |
| Animal Endp Tissue Coll List: | retina, optic nerve |
| Animal Endp Clinical Signs: | See summary |
