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MB Sample ID: SA078178
| Local Sample ID: | C8 |
| Subject ID: | SU001195 |
| Subject Type: | Plant |
| Subject Species: | Brassica nigra |
| Taxonomy ID: | 3710 |
| Age Or Age Range: | 4 weeks old |
| Height Or Height Range: | 15-20 cm |
| Species Group: | Brassicacea |
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Treatment:
| Treatment ID: | TR001210 |
| Treatment Summary: | Two types of treatment were used separately and in sequence: simulated herbivory with use of the phytohormone MeJA (M); real herbivory by P. brassicae caterpillars (P); and combined treatment (MP) where caterpillars were introduced to feed on (M)-plants for five days, after three days of MeJA pre-treatment. MeJA (Sigma-Aldrich, CAS Number 39924-52-2) was applied to the whole plant by spraying 5 mL (1mM, diluted in MilliQ water). First instar caterpillars (15 individuals per plant) were placed on the first fully expanded leaf (L4) of experimental plant and left to feed freely for five days. |
| Plant Light Period: | 16:8 hours light: dark cycle |
| Plant Humidity: | 50-70% RH |
| Plant Temp: | 20-22 °C |
| Plant Growth Stage: | vegetative (4 weeks) |
