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MB Sample ID: SA092254
| Local Sample ID: | 181_1.cdf |
| Subject ID: | SU001338 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
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Treatment:
| Treatment ID: | TR001353 |
| Treatment Summary: | Sample preparation was performed according to A et al (A et al. 2005). In detail, 900 uL of extraction buffer (90/10 v/v methanol:water) including internal standards were added to 100 uL of sample material. The sample was shaken at 30 Hz for 3 minutes in a mixer mill and proteins were precipitated at +4 °C on ice. The sample was centrifuged at +4 °C, 14 000 rpm, for 10 minutes. 200 uL of supernatant were transferred to a micro vial and solvents were evaporated. **** Derivatization was performed according to A et al (A et al. 2005). In detail 30 uL of methoxyamine (15 ug/uL in pyridine) were added to the dry sample and the sample was shaken vigorously for 10 minutes. The reaction was begun by keeping the sample at +70 °C for 1 hour before letting the reaction proceed in room temperature for 16 hours. 30 uL of MSTFA were thenceforth added, the sample was shaken and left to react for 1 hour in room temperature. 30 μL of methyl stearate (15 ng/uL in heptane) were added before analysis. *** REFERENCES A, J., et al. (2005), 'Extraction and GC/MS Analysis of the Human Blood Plasma Metabolome', Anal. Chem., 77 (24), 8086-94. |
| Treatment Protocol Filename: | Analytical_parameters_Sleipner_10m_column_P0287_plasma.pdf |
