Return to study ST001056 main page
MB Sample ID: SA093744
| Local Sample ID: | T1 |
| Subject ID: | SU001363 |
| Subject Type: | Plant |
| Subject Species: | Triticum durum |
| Taxonomy ID: | 4567 |
| Genotype Strain: | cv. Opera |
| Age Or Age Range: | 10 days |
| Gender: | Not applicable |
Select appropriate tab below to view additional metadata details:
Treatment:
| Treatment ID: | TR001378 |
| Treatment Summary: | Seedlings (10 day old) were treated for 0 h (T0), 6 h (T1), 12 h (T2), 24 h (T3) and 96 h (T4) with nitrate (1 mM) and/ or umbelliferone (100 uM) |
| Treatment Protocol Comments: | Durum weath seeds were directly sown in hydroponic systems the 11/March/2018 and allowed to establish for 10 days. Nutritional Regime:Plant were grown in hydroponic solution composed as follow: KNO3 (1 mM); MgSO4 (100 µM); CaSO4 (400 µM); KCl (5 µM); K2SO4 (200 µM); KH h2PO4 (175 µM); H h3BO3 (2.5 µM); MnSO4 (0.2 µM); ZnSO4 (0.2 µM); NaMoO4 (0.05 µM); CuSO4 (0.05 µM); Fe-EDTA (200 µM). Plant Watering Regime: plants were cropped in hydroponic solution daily changed |
| Treatment: | Umbelliferone (Inhibitor: 100 uM), Time (0, 6, 12, 24, 48, 96 h) |
| Treatment Compound: | Umbelliferone (Inhibitor: 100 uM) |
| Treatment Route: | Roots- Hydropony |
| Treatment Dose: | 100 uM |
| Treatment Dosevolume: | Umbelliferone (Inhibitor: 100 uM), Nitrate (1 mM) |
| Treatment Doseduration: | Time (0, 6, 12, 24, 48, 96 h) |
| Treatment Vehicle: | Hoagland Solution |
| Plant Growth Location: | Growth chambers belonging to the University Mediterranea of Reggio Calabria |
| Plant Light Period: | 18h/6h light/dark |
| Plant Humidity: | 60% Relative Humidity |
| Plant Temp: | 25°C |
| Plant Watering Regime: | See protocol comments |
| Plant Nutritional Regime: | See protocol comments |
| Plant Estab Date: | 2018-03-11 |
| Plant Harvest Date: | 2018-03-25 |
| Plant Growth Stage: | Seedlings collected at the formation of second true leaves |
| Plant Metab Quench Method: | Immediately after collection snap frozen in liquid Nitrogen |
| Plant Harvest Method: | Full plants harvested manually (5 plants for replicate) |
| Plant Storage: | Snap frozen and powdered plant material stored at -80°C |
