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MB Sample ID: SA122052
Local Sample ID: | 11A02 |
Subject ID: | SU001511 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
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Treatment:
Treatment ID: | TR001526 |
Treatment Summary: | A total of 20 EBC samples (10 for each group), each 50 µL in volume before concentration, were phenotyped. A pooled sample, which was used as a QC, was prepared by taking 5 µL from each EBC sample and mixing the aliquots together. Then, the 20 samples, together with the pooled QC sample and the blank sample (containing only ultrapure water) were lyophilized at -40 C and 100 mTorr for 24h in a VirTis Benchtop freeze-drier (LP Industries, Stone Ridge, NY, USA). Residues were then reconstituted in 9 μL of methanol/water 1:9 (v:v) with 1×10-6 M 13C-tyrosine spiked in. This resulted in a 5-fold concentration factor. |