Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA125012

Local Sample ID:	Nl_2
Subject ID:	SU001554
Subject Type:	Other
Subject Species:	Nitzschia lecointei;Fragilariopsis cylindrus;Navicula cf. perminuta;Navicula pelliculosa
Taxonomy ID:	186028;186039;908978;913975

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Treatment:

Treatment ID:	TR001569
Treatment Summary:	Antarctic species were grown at −1°C and a PAR irradiance of 45 𝜇mol photons m−2 s−1 (16:8 light:dark cycle) using cool white lights. Temperate species were grown at 13°C and a PAR irradiance of 120 𝜇mol photons m−-2 s−-1(12:12 light:dark cycle). In both cases, light was saturating. Cultures were grown in artificial seawater (ESAW, salinity 31, for Antarctic species and Instant Ocean, salinity ~35 for temperate species). Cobalamin (vitamin B12) was replete in all cultures. To explore the effect of growth conditions on metabolic profiles using non-metric dimensional scaling analysis, samples were included of N. lecointei grown at temperatures of −1 and 4˚C and salinities of 32 and 41.

Treatment ID:

TR001569

Treatment Summary:

Antarctic species were grown at −1°C and a PAR irradiance of 45 𝜇mol photons m−2 s−1 (16:8 light:dark cycle) using cool white lights. Temperate species were grown at 13°C and a PAR irradiance of 120 𝜇mol photons m−-2 s−-1(12:12 light:dark cycle). In both cases, light was saturating. Cultures were grown in artificial seawater (ESAW, salinity 31, for Antarctic species and Instant Ocean, salinity ~35 for temperate species). Cobalamin (vitamin B12) was replete in all cultures. To explore the effect of growth conditions on metabolic profiles using non-metric dimensional scaling analysis, samples were included of N. lecointei grown at temperatures of −1 and 4˚C and salinities of 32 and 41.