Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA138187

Local Sample ID:	37
Subject ID:	SU001710
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

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MS

Treatment:

Treatment ID:	TR001723
Treatment Summary:	MCAD-/- mice were re-derived as germ-free (GF) for the purpose of this study. MCAD-/- mice or MCAD+/+ controls were maintained in sterile gnotobiotic isolators or cages with HEPA-filtered air supply. Urine from GF MCAD-/- and MCAD+/+ mice was collected under sterile conditions. Seven days subsequent, mice were mono-colonized with wild-type C. sporogenes (Cs) by gavage of 200 µL saturated overnight culture. Urine was collected one week after colonization. C. sporogenes colonization was verified with serial dilution plating.

Treatment ID:

TR001723

Treatment Summary:

MCAD-/- mice were re-derived as germ-free (GF) for the purpose of this study. MCAD-/- mice or MCAD+/+ controls were maintained in sterile gnotobiotic isolators or cages with HEPA-filtered air supply. Urine from GF MCAD-/- and MCAD+/+ mice was collected under sterile conditions. Seven days subsequent, mice were mono-colonized with wild-type C. sporogenes (Cs) by gavage of 200 µL saturated overnight culture. Urine was collected one week after colonization. C. sporogenes colonization was verified with serial dilution plating.