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MB Sample ID: SA152066
| Local Sample ID: | 20181008-ND2 |
| Subject ID: | SU001737 |
| Subject Type: | Cultured cells |
| Subject Species: | Plasmodium falciparum |
| Taxonomy ID: | 5833 |
| Genotype Strain: | 3D7 |
| Gender: | Not applicable |
| Cell Counts: | 1x10^8 |
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Treatment:
| Treatment ID: | TR001750 |
| Treatment Summary: | Plasmodium falciparum 3D7 parasites were cultured in RPMI 1640 medium and magnetically enriched to increase the infected to uninfected RBC ratio. Following hemocytometer counts, 1x10^8 parasites were measured per condition into 5 mL of total media for 2.5 hours in the presence of drug. No treatment or ND represents control parasites without additional alterations. Compounds (MMV...) are test compounds that were added at 10xIC50 value for the experimental duration. Blanks are sample tubes that follow the same procedures as samples following the quenching of metabolism. Both Pool and QC samples are combined samples of all samples from the analytical batch on that particular day. Dates in YYYYMMDD format are appended to individual samples to indicate the batch in which they were processed. |
| Treatment Protocol Filename: | Metabolite_Extraction_for_LCMS_2017.pdf |
| Treatment Compound: | MMV084978, MMV019721 |
| Treatment Route: | Transfer to media by pipette |
| Treatment Dose: | 10xIC50 |
| Treatment Dosevolume: | 5 uL or less |
| Treatment Doseduration: | 2.5 hours |
| Treatment Vehicle: | DMSO |
| Cell Storage: | Temperature and gas composition controlled incubator |
| Cell Growth Container: | 6-well plate |
| Cell Growth Config: | 5 mL in each sample well, 1x10^8 infected red blood cells per well, conditions performed in triplicate |
| Cell Media: | RPMI 1640 containing Albumax, Gentamycin, Hypoxanthine, HEPES, Sodium Bicarbonate |
