Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA155391

Local Sample ID:	s1041
Subject ID:	SU001760
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090
Genotype Strain:	Swiss Webster
Age Or Age Range:	10-14 weeks
Gender:	Male

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Treatment:

Treatment ID:	TR001773
Treatment Summary:	Mouse experiments were performed on gnotobiotic Swiss Webster germ-free mice (males, 10-14 weeks of age, n = 3-8 per group for all experiments) maintained in aseptic isolators, and originally obtained from Taconic Bioscience. For mono-association experiments, mice were colonized with Bacteroides thetaiotaomicron VPI 5482, Clostridium sporogenes ATCC 15579, Citrobacter portucalensis BEI HM-34, or Anaerostipes sp. BEI HM-220904a, by oral gavage (200 uL, ~1 x 107 CFU) and were maintained on a standard chow (LabDiet 5K67). For the defined-community experiment, mice with a six-member community were colonized with a 200 uL mixture consisting of equal volumes from saturated cultures of Bacteroides thetaiotaomicron VPI 5482 (8.7 x 109 CFU), Clostridium sporogenes ATCC 15579 (1.4 x 108 CFU), Edwardsiella tarda ATCC 23685 (3.6 x 1010 CFU), Collinsella aerofaciens ATCC 25986 (1.4 x 109), Eubacterium rectale ATCC 33656 (6.9 x 106 CFU), and Parabacteroides distasonis ATCC 8503 (1.5 x 109 CFU). Mice with a five-member community were colonized with all cultures mixed at the same volumes as described above except for Clostridium sporogenes ATCC 15579, which was not included. Successful colonization and stable community members were determined by 16S amplicon sequencing of the V4 (515f, 806r) region of microbial populations present in the feces and cecal contents from individual mice.

Treatment ID:

TR001773

Treatment Summary:

Mouse experiments were performed on gnotobiotic Swiss Webster germ-free mice (males, 10-14 weeks of age, n = 3-8 per group for all experiments) maintained in aseptic isolators, and originally obtained from Taconic Bioscience. For mono-association experiments, mice were colonized with Bacteroides thetaiotaomicron VPI 5482, Clostridium sporogenes ATCC 15579, Citrobacter portucalensis BEI HM-34, or Anaerostipes sp. BEI HM-220904a, by oral gavage (200 uL, ~1 x 107 CFU) and were maintained on a standard chow (LabDiet 5K67). For the defined-community experiment, mice with a six-member community were colonized with a 200 uL mixture consisting of equal volumes from saturated cultures of Bacteroides thetaiotaomicron VPI 5482 (8.7 x 109 CFU), Clostridium sporogenes ATCC 15579 (1.4 x 108 CFU), Edwardsiella tarda ATCC 23685 (3.6 x 1010 CFU), Collinsella aerofaciens ATCC 25986 (1.4 x 109), Eubacterium rectale ATCC 33656 (6.9 x 106 CFU), and Parabacteroides distasonis ATCC 8503 (1.5 x 109 CFU). Mice with a five-member community were colonized with all cultures mixed at the same volumes as described above except for Clostridium sporogenes ATCC 15579, which was not included. Successful colonization and stable community members were determined by 16S amplicon sequencing of the V4 (515f, 806r) region of microbial populations present in the feces and cecal contents from individual mice.