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MB Sample ID: SA155496
Local Sample ID: | HFD-PBS_3.4.4 |
Subject ID: | SU001764 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57Bl/6N |
Age Or Age Range: | 35 weeks |
Gender: | Male |
Animal Animal Supplier: | KU Leuven Animal Laboratory |
Animal Feed: | control diet (E15742-33 ssniff Spezialdiäten GmbH) or 60% high fat diet (S8655-E220 sniff Spezialdiäten GmbH) |
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Treatment:
Treatment ID: | TR001777 |
Treatment Summary: | 2-week old mice were injected i.p. diethylnitrosamine (DEN, 25 mg/Kg) in phosphate buffered saline (3.17 mg/ml). At 6-weeks of age, mice were randomized into two groups: control diet (CD, E15742-33 ssniff Spezialdiäten GmbH) or high fat diet (HFD, S8655-E220 sniff Spezialdiäten GmbH). |
Treatment Compound: | dietylnitrosamine (DEN) |
Treatment Route: | i.v. |
Treatment Dose: | 25mg/kg |
Treatment Doseduration: | single dose at 2-weeks age |
Treatment Vehicle: | phosphate buffered saline (PBS) |
Animal Endp Euthanasia: | 50 µl of 60 mg/ml Nembutal solution (Vetoquinol) |
Animal Endp Clinical Signs: | 20% increase in abdomen diameter and hard mass detection via palpation, loss of ambulation, loss of skin elasticity, labored respiration, or weight loss over 20 % of initial body weight |