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MB Sample ID: SA185347

Local Sample ID:	pooled sample QC 1/3 dilution
Subject ID:	SU002056
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090
Gender:	Male and female

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Treatment:

Treatment ID:	TR002068
Treatment Summary:	Control AAV-GFP, MTX, AAV-SOD3 + buffers from Study Design. The pAAV-IRES-hrGFP vector was purchased from Agilent Technologies (240075) and pcDNA3.1-myc HisA (-)/ human SOD3 (myc-his tag at C-terminal) (36). Virus production and purification were performed by the Viral Core at BCH according to standard procedures (37). CD-1 mice (6-8 weeks) or Sprague Dawley rats (8 weeks) were given a single intravenous injection of 75 mg/kg MTX (Fisher Scientific, AAJ66364MD) dissolved in 0.9 % NaCl (Mountainside Medical, 0409-4888-10). MTX was freshly prepared for each experiment. MTX in mouse serum or CSF was assessed by MTX ELISA according to the manufacturer's instructions (Enzo Life Sciences, 142-0001).

Treatment ID:

TR002068

Treatment Summary:

Control AAV-GFP, MTX, AAV-SOD3 + buffers from Study Design. The pAAV-IRES-hrGFP vector was purchased from Agilent Technologies (240075) and pcDNA3.1-myc HisA (-)/ human SOD3 (myc-his tag at C-terminal) (36). Virus production and purification were performed by the Viral Core at BCH according to standard procedures (37). CD-1 mice (6-8 weeks) or Sprague Dawley rats (8 weeks) were given a single intravenous injection of 75 mg/kg MTX (Fisher Scientific, AAJ66364MD) dissolved in 0.9 % NaCl (Mountainside Medical, 0409-4888-10). MTX was freshly prepared for each experiment. MTX in mouse serum or CSF was assessed by MTX ELISA according to the manufacturer's instructions (Enzo Life Sciences, 142-0001).