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MB Sample ID: SA189687
| Local Sample ID: | 20191120-BKE2_glucose-3 |
| Subject ID: | SU002106 |
| Subject Type: | Cultured cells |
| Subject Species: | Plasmodium falciparum |
| Taxonomy ID: | 5833 |
| Genotype Strain: | NF54 attB |
| Gender: | Not applicable |
| Cell Counts: | 1x10^8 |
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Treatment:
| Treatment ID: | TR002118 |
| Treatment Summary: | Plasmodium falciparum NF54 attB parasites were cultured in RPMI 1640 medium and magnetically enriched to increase the infected to uninfected RBC ratio. Following hemocytometer counts, 1x10^8 parasites were measured per condition into 5 mL of total media for 2.5 hours in the presence of the carbon-13 metabolite (glucose, glutamine, or acetate). Wild-type or WT represents control parasites without additional alterations. Blanks are sample tubes that follow the same procedures as samples following the quenching of metabolism. Both Pool and QC samples are combined samples of all samples from the analytical batch on that particular day. Dates in YYYYMMDD format are appended to individual samples to indicate the batch in which they were processed. |
| Treatment Protocol Filename: | Metabolite_Extraction_for_LCMS_2017.pdf |
| Treatment Compound: | Universally labelled glucose, glutamine, or acetate |
| Treatment Route: | Transfer to media by pipette |
| Treatment Dosevolume: | 50 uL (glucose), 25 uL (glutamine), or 10 uL (acetate) |
| Treatment Doseduration: | 2.5 hours |
| Treatment Vehicle: | RPMI 1640 media (lacking the respective metabolite) |
| Cell Storage: | Temperature and gas composition controlled incubator |
| Cell Growth Container: | 6-well plate |
| Cell Growth Config: | 5 mL in each sample well, 1x10^8 infected red blood cells per well, conditions performed in triplicate |
| Cell Media: | RPMI 1640 containing Albumax, Gentamycin, Hypoxanthine, HEPES, Sodium Bicarbonate |
