Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA209029

Local Sample ID:	23
Subject ID:	SU002261
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090
Age Or Age Range:	8 - 10 weeks
Gender:	Male

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Treatment:

Treatment ID:	TR002273
Treatment Summary:	Male 8 – 10 week old C57BL/6 mice were obtained from Charles River Laboratories (Frederick, MD, USA) and were irradiated using the FLASH irradiator at the Radiological Research Accelerator Facility [16] (Figure S1). This novel irradiator is based on a Clinac 2100C (Varian Medical Systems, Corona, CA, USA) where the pulse delivery is controlled using in house software. All irradiations were performed using 9 MeV electrons with no scatterer or flattening filter. For these experiments, mice were placed in a 72 mm x 41mm x41mm acrylic box in which air holes had been drilled (The Container Store, Coppell, TX, USA). For 0.7 Gy/min irradiations, mice (n=6) were individually placed at 120 cm above the clinac head and irradiation delivered at 3.25 pulses per second. In this configuration, 3 Gy was delivered in 580 pulses. For 7 Gy/sec mice (n=6) were individually placed 20cm above the clinac head (Figure S1) and dose delivered at 180 pulses/sec after allowing 20 sec where the acceleration and electron source were both on but operated asynchronously so that no beam is delivered. In this configuration, 3 Gy was delivered in 78 pulses. Dosimetry was performed prior to irradiation using a NIST-traceable Advanced Marcus Ion Chamber (AMIC) and Unidos E electrometer (PTW, Freiburg, Germany). Verification of dosimetry was performed using OBT3 radiochromic film (Ashland Specialty Chemicals, Wayne, NJ, USA).

Treatment ID:

TR002273

Treatment Summary:

Male 8 – 10 week old C57BL/6 mice were obtained from Charles River Laboratories (Frederick, MD, USA) and were irradiated using the FLASH irradiator at the Radiological Research Accelerator Facility [16] (Figure S1). This novel irradiator is based on a Clinac 2100C (Varian Medical Systems, Corona, CA, USA) where the pulse delivery is controlled using in house software. All irradiations were performed using 9 MeV electrons with no scatterer or flattening filter. For these experiments, mice were placed in a 72 mm x 41mm x41mm acrylic box in which air holes had been drilled (The Container Store, Coppell, TX, USA). For 0.7 Gy/min irradiations, mice (n=6) were individually placed at 120 cm above the clinac head and irradiation delivered at 3.25 pulses per second. In this configuration, 3 Gy was delivered in 580 pulses. For 7 Gy/sec mice (n=6) were individually placed 20cm above the clinac head (Figure S1) and dose delivered at 180 pulses/sec after allowing 20 sec where the acceleration and electron source were both on but operated asynchronously so that no beam is delivered. In this configuration, 3 Gy was delivered in 78 pulses. Dosimetry was performed prior to irradiation using a NIST-traceable Advanced Marcus Ion Chamber (AMIC) and Unidos E electrometer (PTW, Freiburg, Germany). Verification of dosimetry was performed using OBT3 radiochromic film (Ashland Specialty Chemicals, Wayne, NJ, USA).