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MB Sample ID: SA210287

Local Sample ID:HUPA_93
Subject ID:SU002280
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

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Treatment:

Treatment ID:TR002292
Treatment Summary:Plasma samples were inactivated with cold (-20°C) MeOH : EtOH (1:1, v/v) and vortex-mixed for 1 min, incubated on ice for 5 min, and centrifuged for 20 min at 16000 xg at 4°C. The resulting supernatant was stored at -80°C until analysis. Due to the broad differences in physical-chemical properties of metabolites, we used GC-MS (focused on small molecules that can be made volatile by derivatization) and CE-MS (focused on polar and ionic compounds) in order to increase the metabolite coverage. Sample preparation for GC-MS and CE-MS was detailed in the uploaded file in sample preparation part.
Treatment Protocol Filename:Sample_treatment.pdf
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