Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA249750

Local Sample ID:	MED1 Condensate Sample 6
Subject ID:	SU002592
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

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Treatment:

Treatment ID:	TR002604
Treatment Summary:	Mouse liver metabolites were combined with either the condensate-forming low-complexity domains of HNRNPA1, MED1 or full-length SARS-CoV-2 nucleocapsid. Condensates were stimulated with either 0 nM, 150 nM or 600 nM RNA. Condensates were centrifuged to the bottom of a 600 ul tube. Equal fractions from the input sample, aqueous phase and condensate phases were collected separately. Metabolites were extracted from each fraction. Alternatively, liver metabolites were added 10 min after stimulating condensate formation with phage RNA and incubating for 2 min prior to centrifugation. In a separate set of experiments, the extraction procedure from the three fractions (ie aqueous, condensate and input) was altered to remove a heat step.

Treatment ID:

TR002604

Treatment Summary:

Mouse liver metabolites were combined with either the condensate-forming low-complexity domains of HNRNPA1, MED1 or full-length SARS-CoV-2 nucleocapsid. Condensates were stimulated with either 0 nM, 150 nM or 600 nM RNA. Condensates were centrifuged to the bottom of a 600 ul tube. Equal fractions from the input sample, aqueous phase and condensate phases were collected separately. Metabolites were extracted from each fraction. Alternatively, liver metabolites were added 10 min after stimulating condensate formation with phage RNA and incubating for 2 min prior to centrifugation. In a separate set of experiments, the extraction procedure from the three fractions (ie aqueous, condensate and input) was altered to remove a heat step.